Figure 2
From: Developmental control of lateralized neuron size in the nematode Caenorhabditis elegans
ASEL/ASER nucleoli number, but not nucleus size or DNA content, is lateralized. (A) Measurement of nuclei sizes in ASEL and ASER using a nuclear-tagged che-1fosmid::yfp (otIs188). Error bars are standard error of the mean (s.e.m.); ns, not significant. A representative pair of nuclei images from one worm is shown. (B) Ratio of ploidy in ASEL and ASER. Ploidy was measured by relative DAPI intensity in worms containing a che-1prom::mChOpti transgene that labels ASE neurons in red. Error bars are s.e.m.; ns, not significant. A representative pair of DAPI images from one worm is shown. (C) Measurement of number of nucleoli per cell in ASEL and ASER using an antibody targeting FIB-1. ASE neurons were identified with a che-1prom:: mChOpti transgene that labels ASE neurons in red. An example image of a worm head is shown; positions of ASEL and ASER are indicated with dashed circles, and arrows point to FIB-1 nucleoli foci. **P < 0.02, as determined by a Wilcoxon signed-rank test. (D) Measurement of number of nucleoli per cell in ASEL and ASER using a translational FIB-1::GFP reporter [52]. ASE neurons were identified with a che-1prom:: mChOpti transgene that labels ASE neurons in red. An example image of a worm head is shown, as in (B). *P < 0.05, as determined by a Wilcoxon signed-rank test.