Figure 7

Ectopic Numb5 and Numb6 expression alters cell migration in vitro and in vivo. (A) Migration of neural crest cells expressing Numb5-GFP or Numb6-DsRed in vitro at 24 hours using the Boyden chamber. NUMB5-GFP or NUMB6-DsRed expressing neural crest cells demonstrate significantly higher migration than their GFP or NUMB2-GFP expressing counterparts. Dominant negative (dn) CDC42 and, to a lesser degree, NUMB2 mutation (NUMB2/S264A) tend to retain normal migration. Data are presented as means ± standard deviation using three experiments originating from discrete infections. (B) In vivo migration analysis of neural crest cells expressing Numb5-GFP eletroporated into stage 4 chick embryos. Numb5-GFP over-expression leads to irregular abrogated neural crest migration in the chick embryos. The confocal images of stage 4 chick embryos were taken 12 hours after GFP or Numb5-GFP electroporation into the dorsal region. Green shows GFP expression; DAPI indicates nuclei staining.