Skip to main content
Figure 5 | Neural Development

Figure 5

From: Two novel human NUMB isoforms provide a potential link between development and cancer

Figure 5

Numb5 and Numb6 enhance lamellipodial and filopodial growth, respectively. (A) Morphological analyses of cytoplasmic protrusion formation in N2a cells expressing Numb5-GFP and Numb6-GFP. (B) Zymographic detection of MMP2 and MMP9 gelatinases secreted to conditioned media in DB7 (upper panel) and Met-1 (lower panel) cells over-expressing GFP, Numb4, Numb5, and Numb6. (C) Western blot analyses of Cdc42 and Rac1 protein expression in N2a cells over-expressing Numb6-GFP. (D) Confocal immunofluoresence analysis of filopodial growth regulation by Cdc42 (upper panel) and Rac1 (lower panel) GTPases in N2a cells over-expressing Numb6-GFP. The Numb6 induced filopodial growth is significantly abrogated by dominant negative (dn) Cdc42 and dominant negative (dn) Rac1. Scale bar = 10 μm.

Back to article page