Figure 4
From: Two novel human NUMB isoforms provide a potential link between development and cancer
NUMB5 and NUMB6 expression effects Notch signaling. (A) RT-PCR analysis of mRNA extracted from brain glioblastoma U87 cells. Negative control, no template; loading control, β-actin. (B) RT-PCR analysis of JAG1 and JAG2 in the HEK293GPG cells infected with NUMB4, NUMB5 or NUMB6. Negative control, no template; control, no infection; loading control, β-actin. (C) Luciferase assay of Notch activation by Delta in N2a cells expressing NUMB isoforms using CSL-luciferase vector. Data were obtained from five independent experiments (in quadruplicate), and are presented as mean + standard deviation. *P < 0.025.