Figure 3
From: Two novel human NUMB isoforms provide a potential link between development and cancer
Numb5 and Numb6 expression in tumor development. (A) Schematic of the over-expression plasmid used for MMTV-NUMB6-DsRed transgenic mouse production. (B) Schematic of the Cre recombinase over-expression plasmid used for NUMB5-EGFP transgenic mouse production. (C) Immunohistochemistry analysis of retinoblastoma (Rb) and p53 in the brain and retinal tumors. Green indicates Numb5 transgenic over-expression, and red Rb or p53 antibody detection; yellow shows co-localization of the two proteins. Blue visualizes Dapi staining of the nucleus. (D) Immunohistochemistry analysis of the Notch intracellular domain and p53 in the retinal tumor. Red shows Numb6 expression in tissue derived from MMTV transgenic mouse. Green shows Notch or p53 Immunofluorescence detection. Yellow indicates co-localization of the two proteins. Blue is Dapi staining of the nucleus. LTR, long-terminal repeat; PDGF, platelet-derived growth factor.