Figure 1

The ephrinA5-p75^NTR interaction is promoted in a ligand-dependent manner. CHO cells were transfected with cDNA expression vectors for p75^{NTR, FLAG} and ephrinA5^HA. A day later cells were serum starved and treated for 30 minutes with 100 ng/ml NGF as indicated. Subsequently, cells were lysed and immunoprecipitated using a αHA antibody. Western blot analyses of input and immunoprecipitate showed that co-immunoprecipitation of p75^NTR and ephrinA5 is increased in the presence of ligand. A quantification of these experiments and comparable experiments using proneurotrophins are shown in Additional files 1 and 2. The asterisk marks the Ig heavy chain of the antibody used for immunoprecipitation. IB, immunoblot; IP, immunoprecipitation.