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Figure 4 | Neural Development

Figure 4

From: Brn3a regulates neuronal subtype specification in the trigeminal ganglion by promoting Runx expression during sensory differentiation

Figure 4

Early defects in specification of TrkC neurons in the Brn3a-/- TG. The TG of Brn3a knockout and control embryos were examined at various stages for the expression of TrkB, TrkC and Runx3. (A-D) At E10.5 TrkB and TrkC are extensively co-expressed in both genotypes. Runx3 was not detected at this stage in either genotype. (E-H) At E12.5 TrkB and TrkC expression identify discrete subsets of neurons in control ganglia (E), but in Brn3a knockout ganglia co-expression persists (F), and examples of the numerous co-expressing neurons are indicated by arrowheads. Runx3 and TrkC are co-expressed in control ganglia (G), but Runx3 expression is not initiated in the knockout TG, and TrkC expression is diminished (H). (I-L) At E13.5, TrkB expression is markedly expanded in knockout ganglia (J), and TrkC and Runx3 expression is nearly absent (J, L). Rare remaining TrkC+/Runx3+ neurons in the knockout TG are indicated by arrows (J, L). (M-P) Early Ret+ neurons at this stage do not co-express TrkA or Runx1 in the control or knockout ganglia, and persist in Brn3a-/- ganglia. Ret+ neurons at this stage are likely to represent a subset of mechanoreceptors; Ret+ nociceptors are not detected until the perinatal period. Scale bar = 50 μM.

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