Figure 5
From: Ihog and Boi are essential for Hedgehog signaling in Drosophila
ihog and boi are required for Smo accumulation. All panels show third instar wing imaginal discs with anterior to the left. (A-B'') In controls, ihogDC1/DC1clones (dotted line) were made in a boiC1/+heterogygote. (A-A'') Low magnification view. (B-B'') Higher magnification view of boxed area in (A''). Anti-Smo immunoreactivity (red) is normally enriched in the posterior versus anterior compartment, but highest accumulation of Smo occurs in Hh-responsive anterior cells immediately adjacent to the compartment boundary. Within the control clone indicated, Smo levels were unaffected. (C-D'') An ihogDC1/DC1clone (dotted line) in a boiC1/-mutant. (C-C'') Low magnification view. (D-D'') Higher magnification view of boxed area in (C''). Within clones lacking both ihog and boi, Smo staining levels were markedly reduced.