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Figure 2 | Neural Development

Figure 2

From: RBP-J is not required for granule neuron progenitor development and medulloblastoma initiated by Hedgehog pathway activation in the external germinal layer

Figure 2

Deletion of Patched1 and RBP-J in granule neuron progenitors results in the expression of Hedgehog pathway target genes and loss of Notch pathway target genes. (A-D) The universal Hedgehog target Gli1 is expressed in control GNPs as well as in the EGL of all mutants at postnatal day 7, as shown by in situ analysis. (E-H) Immunofluorescence staining of P7 brain tissue shows the canonical Notch target Hes1 is expressed in the outer EGL in RBP-J deleted GNPs (G), comparable to controls (E) and the number of Hes1 expressing cells in the EGL of Ptc1 deleted (F) and Ptc1 and RBP-J deleted brains is increased (H). (I,J) Hedgehog activation in GNPs slightly increases the area of Hes5 mRNA expression (J) compared to controls (I). (K,L) In situ hybridisation for the canonical Notch target Hes5 confirms loss by deletion of RBP-J (K) in P7 brains and loss of Hes5 in most but not all GNPs of Ptc1lox/lox;RBP-Jlox/lox;Math1-Cre tumours (L). Images in each panel were taken at the same magnification. Counter stain for in situ hybridisation was nuclear fast red and for immunofluorencence DAPI. Scale bars represent 50 μm for all images. ML, molecular layer.

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Neural Development

ISSN: 1749-8104