Drosophilatype II neuroblast lineages keep Prospero levels low to generate large clones that contribute to the adult brain central complex

Table 2 Analysis of 9D11 expression in wild-type and prospero misexpression brains

Genotype and stage	GFP+ INP^a	GFP+ type II progeny^b	Sample size^c
9D11-G4, UAS-nls-GFP @ 25°C
24 h ALH	12.4 ± 3.5	27 ± 5.8	8
48 h ALH	18.1 ± 2.4	52.6 ± 9.7	8
72 h ALH	51.7 ± 4.4	142.2 ± 6.6	13
96 h ALH	80.1 ± 6.2	232.7 ± 6.2	10
9D11-G4, UAS-mCD8::GFP @ 25°C
24 h ALH	11.8 ± 5.8	25.6 ± 5.8	5
48 h ALH	16.11 ± 5.5	53.44 ± 18.3	8
72 h ALH	52.1 ± 10.3	527.5 ± 24.4	9
96 h ALH	83.5 ± 2.5	548.9 ± 14.9	7
9D11-G4, UAS-mCD8::GFP @ 30°C
96 h ALH	86.6 ± 6.3	619.3 ± 20.1	7
120 h ALH	710.8 ± 14.1	97.6 ± 2.3	5
9D11-G4, UAS-mCD8::GFP, UAS-Pros @ 30°C
96 h ALH	14.2 ± 2.8	197.6 ± 20.6	10
120 h ALH	18.6 ± 2.8	214.8 ± 18.3	8

ISSN: 1749-8104