Drosophilatype II neuroblast lineages keep Prospero levels low to generate large clones that contribute to the adult brain central complex

Table 1 Analysis of 19H09 expression in wild-type and prospero misexpression brains

Genotype and stage	Type II NB^a	GFP+ type II NB^b	GFP+ type I NB^c	GFP+ INP^d	GFP+ type II progeny^e	Sample size^f
19H09-G4, UAS-nls-GFP @ 25°C
96 h ALH	8.0	4.6 ± 0.5	1.9 ± 1.3	18.9 ± 5.9	58.9 ± 17.4	7
120 h ALH	8.0	5.4 ± 0.5	6.6 ± 1.9	62.9 ± 6.4	138.1 ± 14.1	8
19H09-G4, UAS-mCD8::GFP @ 25°C
96 h ALH	8.0	5.5 ± 0.7	1.9 ± 1.4	31.7 ± 5.9	84 ± 16.3	10
120 h ALH^g	8.0	6.5 ± 0.8	8.3 ± 1.5	81.9 ± 9.0	232.5 ± 14.2	8
19H09-G4, UAS-mCD8::GFP @ 30°C
120 h ALH^g	8.0	5.8 ± 0.4	7.0 ± 1.8	75.5 ±3.7	242.7 ± 34.5	6
19H09-G4, UAS-mCD8::GFP, UAS-pros @ 30°C
120 h ALH	7.3 ± 0.5	4.6 ± 0.7	7.3 ± 1.5	9.8 ± 2.6	108.8 ± 13.5	9

^aLarge Dpn+ Ase-. ^bLarge GFP+ Dpn+ Ase-. ^cLarge GFP+ Dpn+ Ase+. ^dSmall GFP+ Dpn+. ^eSmall GFP+ cells in type II lineages. ^fBrain lobes. ^gAt 120 h ALH, 19H09-driven mCD8::GFP weakly labels one or two extra type II lineages.

ISSN: 1749-8104