Figure 5

Lineage tracing with 9D11 labels the adult central complex and associated regions. (A,B) 9D11 expression in the adult brain stained for mCD8 (white) and synaptic marker nc82 (magenta). (A,A') Frontal (A) and sagittal (A') views of the three-dimensional reconstruction of mCD8::GFP confocal z-stacks, close up on the FB. Three cell pairs are located dorsal to the FB while two cell pairs are more ventral at the level of the dorsal FB (asterisks). The projections from the dorsal cell pairs enter the dorsal FB at medial sites (green arrowhead) while the projections of ventral pairs enter the dorsal FB at more anterolateral sites (white arrowhead). See Additional file 5 for the three-dimensional reconstruction. (A'') Single frontal confocal section of the same brain at the level of the FB. (B-B''') Serial higher magnification frontal confocal sections of the central complex from posterior to anterior. Cell bodies are posterior to (B). White brackets indicate the dense dorsal layer of innervations at the FB. White outlines represent the neuropils visualized by nc82 staining (labeled). (C-G) Lineage tracing with 9D11-Gal4 in the adult brain stained for GFP (white) and nc82 (magenta). (C,C') Frontal (C) and dorsal (C') views of the three-dimensional reconstruction of GFP confocal z-stacks. (C) Most cell bodies in the posterior cortex were removed for easier viewing. (D-G) Serial frontal confocal sections of the same brain from posterior to anterior show labeling at the PB and cell bodies in the posterior brain (D), the FB and NO (E), the anterior FB, EB, LALs, and BUs (F), and the LALs and middle brain (G). CA, calx. VBC, ventral body commissure. See Additional file 6 for more representative stacks, Additional file 8 for high magnification images of the central complex, and Additional file 7 for the three-dimensional reconstruction. Orientation: d, dorsal; v, ventral; p, posterior; l, lateral. Scale bars: 40 μM.