Figure 3

Prospero misexpression in type II NBs reduces lineage size but does not induce type I NB identity. (A,B,D-H) High magnification confocal images of type II NBs and associated progeny from third instar larval brains expressing mCD8::GFP (A,D) or mCD8::GFP and Pros (B,E-H) under control of 19H09-Gal4. White outlines represent the GFP labeled areas. Type II NBs are indicated with white arrows and mature INPs are indicated with green arrows. (C) Histogram showing number of INPs and total cells labeled by mCD8::GFP driven by 19H09-gal4 in control and pros overexpression brains at 120 h ALH. Error bars indicate standard deviation. (I,I') Three-dimensional reconstruction of medial (I) and lateral (I') views of a 120 h ALH brain lobe expressing mCD8::GFP and Pros under control of 19H09-Gal4. Type II lineages and their axonal projections are showm in white, and the mushroom body, visualized by FasII, is shown in magenta. The optic lobe is removed and the brain cropped for a clearer view. Brains are in the orientations shown in the insets, with imaged lobes indicated with a white dashed line and their mushroom bodies shown. The white arrow points at a split axon tract from a type II lineage. Orientation: d, dorsal; v, ventral; p, posterior; l, lateral; m, medial. Scale bars: (A-E) 10 μM; (F,F') 40 μM.