Figure 2

9D11 specifically labels INPs and their progeny within the central brain. (A,B,D-F) Confocal images of first (A) and third (B,D,E,F) instar larval brains expressing nls-GFP (A,B) or mCD8::GFP (D-F) under 9D11-Gal4 stained for indicated markers (white boxes). Low magnification images of single brain lobes are presented in (A-F), and high magnification images of boxed areas are presented in (A'-F'), respectively. White outlines represent the GFP-labeled areas; yellow outlines represent NB lineages visualized by Discs-large (Dlg) staining. Type II NBs are indicated with white arrows; Ase- Dpn- immature INPs next to type II NBs are indicated with asterisks; Ase+ Dpn- immature INPs close to type II NBs are indicated with arrowheads. Mature INPs are small Dpn+ cells in white outlined areas or indicated with yellow arrows. (E) Green arrows point at the two lateral type II NBs. (C) Histogram showing increasing number of INPs and total cells labeled by nls-GFP driven by 9D11-gal4 between 24 and 96 h ALH. Error bars indicate standard deviation. (G,G') Three-dimensional reconstruction of medial (G) and lateral (G') views of a 120 h ALH brain lobe expressing mCD8::GFP under control of 9D11-Gal4. Type II lineages and their axonal projections are shown in white, and the mushroom body, visualized by FasII, is shown in magenta. The optic lobe is removed and the brain cropped for a clearer view. See text for lineage labels. Brains (gray outline) are in the orientations shown in the insets, with imaged lobes indicated with a white dashed line and their mushroom bodies shown, same as in Fig. 1. White arrows point at commissural projections and arrowheads point at descending ipsilateral projections from type II lineages. The yellow arrow points at the dorsoposterior commissure. Type II lineages were labeled according to [12]. Orientation: d, dorsal; v, ventral; p, posterior; l, lateral; m, medial. Scale bars: (A) 20 μM; (B-F) 50 μM; (A'-F') 10 μM; (G,G') 40 μM. CM, centromedial lineages. DPMm, medial dorsoposterior lineages, medial subgroup. DPMpm, medial dorsoposterior lineages, posteromedial subgroup.