Table 1 Primary antibodies used in this study
Antibody | Type | Dilution | Clone | Source | Immunogen | Characterization of specificity (reference) |
|---|---|---|---|---|---|---|
Complexin 3 | Rb pc | 1:10,000 | Â | Synaptic Systems cat#122302 | Full-length mo complexin 3 | Immunofluorescence of heterologous cells expressing complexin isoforms; western blot (this study) |
Complexin 4 | Rb pc | 1:75,000 | Â | Synaptic Systems cat#122402 | Full-length mo complexin 4 | Immunofluorescence of heterologous cells expressing complexin isoforms; immunofluorescence of morphant visual system (this study) |
zpr 1/FRet 43 | Mo mc | 1:400 | Â | ZIRC | Adult zf retinal cells | Immunofluorescence of zf retina [31] |
zn 1 | Mo mc | 1:40 | Â | ZIRC | Homogenized 1- to 5-dpf whole zf or membrane or basal lamina fractions | Immunofluorescence of zf inner ear [41] |
PKC | Mo mc | 1:1,000 | MC5 | BD Biosciences Pharmingen cat#554207 | Bovine full-length PKC | Immunofluorescence of gf retina [32] |
myc | Mo mc | 1:250 | 9E10 | Zymed Laboratories cat#13-2500 | Hu c-myc amino acids 408-439 | Antibody reacts specifically to hu c-myc amino acids 410-419 (manufacturer) |
RIBEYE b | Rb pc | 1:500 | Â | Dr T Nicolson | Zf RIBEYE b amino acids 133-483 | Immunofluorescence of zf hair cells [36] |
CtBP 2 | Mo mc | 1:1,000 | 16/CtBP 2 | BD Biosciences Pharmingen cat#612044 | Mo CtBP 2 amino acids 361-445 | Western blot with retinal homogenates reveals expected bands of 120 and 110 kDa [15] |