Figure 2

Exon-intron organization of orthologous mammalian and zebrafish cplx 3 and cplx 4 genes and their syntenic relationships. (A) The gene structures for human, mouse, and some zebrafish cplx 3 and 4 orthologs are shown. Exons are denoted by yellow boxes, while introns are indicated with connecting lines. The lengths of the exons and introns are drawn to scale, except for human and mouse cplx 4. Cplx 3/4 gene structure is highly conserved, with three exons interrupted by two introns. The length of the second exon, which encodes part of the putative SNARE-binding domain, is exactly 88 nucleotides in all species examined. (B) Syntenic relationships of zebrafish, human, and mouse cplx 3/4 orthologs are shown. The genomic architectures of mammalian cplx 3 orthologs and mammalian cplx 4 orthologs are conserved. Human and mouse cplx 3 are preceded by CSK and LMAN1L/ERGIC-53L, while human and mouse cplx 4 are bounded by LMAN1/ERGIC-53 and RAX. Zebrafish cplx 3a, which has been mapped to chromosome 25, is preceded by a zebrafish ortholog of CSK. Zebrafish cplx 4a is bordered by LMAN and RX3, which is the zebrafish ortholog of mammalian RAX. Ada, adenosine deaminase; chr, chromosome; cplx, complexin; CSK, c-src kinase; ERGIC-53, ER Golgi intermediate compartment 53-kDa; ERGIC-53L, ERGIC-53, ER Golgi intermediate compartment 53-kDa-like; hu, human; LMAN1, lectin mannose-binding 1; LMAN1l, lectin mannose-binding 1-like; mo, mouse; RAX, retina and anterior neural fold homeobox gene; rx3, retinal homeobox gene 3; zf, zebrafish.