Figure 9

Orientation of the Golgi apparatus in Purkinje cells of E14.5 normal and reeler mice. (A, C) Purkinje cells initiating the formation of the Purkinje plate (PP) in an outer region of normal cerebella (A) and those accumulating in the corresponding region of reeler cerebella without forming the PP (C) were analyzed for their Golgi orientation using anti-giantin and anti-Lhx1/5 immunostaining. (B) Graphs depicting the relative position of Lhx1/5⁺ nuclei examined within a range of 70 μm from the pial surface (in increments of 10 μm), showing the existence of a cell-sparse zone (bins 6 and 7; asterisks in (A)) that demarcates pially arranged nuclei (bins 3 to 5) as a plate in +/rl cerebella. (D-F) Golgi orientation relative to the nucleus (cells A1 and A2 from (A); cells C1 and C2 from (C)), with the results summarized in (E) (normal cerebella) and (F) (reeler cerebella). Golgi orientation towards the pial side (from 'XI o'clock' to 'I o'clock') was observed more frequently in the normal group than in the reeler group (Chi-square test, P < 0.001). In reeler cerebella, Golgi orientation was more posterior than in normal cerebella (Chi-square test, P < 0.01). Cells were counted in four independent sections from two embryos in each group (+/rl or rl/rl).