Figure 7

Comparison of axonogenic and migratory behaviors of nascent Purkinje cells between normal and reeler cerebella at E13.5. (A-C) The expression pattern of Corl2 and Neurofilament (A and B) and the position of E10.5-adenovirally labeled GFP⁺ cells (C) are indistinguishable between normal (+/rl) and reeler (rl/rl) cerebella at E13.5 (each comparison was made between littermates). (D, E) Normal axonogenic behavior of E10.5-born Purkinje cells in reeler cerebella until E13.5 is also suggested by traces of individual GFP⁺Lhx1/5⁺ cells. (E) An E10.5-born Purkinje cell observed in an E13.5 reeler cerebellar section. Note that this cell successfully migrated to an outer cerebellar region with an anteriorly extended axon-like fiber. Non-axon-like thick processes (as seen in the posterior or ventricular pole of Purkinje cells in normal E13.5 cerebella (Figure 2Q, R; Additional file 2D)) are not observed.