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Figure 3 | Neural Development

Figure 3

From: Migration, early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior-born Purkinje cells in the developing mouse lateral cerebellum

Figure 3

Triple immunolabeling analysis to demonstrate the relationship between tangentially oriented nascent Purkinje cells and radial fibers. (A-G, K, L) Sections as used for the E10.5 to E12.5 tracing analysis (Figure 2A-I) were stained with anti-GFP (green), anti-Lhx1/5 (cyan), and anti-Nestin (red). In each of the three representative cases, magnified views for the somal part (open arrowhead) are provided to confirm Lhx1/5 immunoreacivity. (H-L) In case 3, three-dimensional reconstructions are shown.

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