Figure 1

Stage-dependent changes of the overall distribution of Purkinje cells in embryonic cerebella demonstrated with Lhx1/5 immunohistochemistry and adenovirus-mediated pulse-chase methods. (A-D) Anti-Lhx1/5 immunostained sagittal sections of normal (ICR or +/rl) (A, C) and reeler (rl/rl) (B, D) cerebella. Sections in each row of (A, B) are arranged in a lateral-to-medial order. The dorsal side is on the top of each picture and the posterior side is on the right. At E14.5, the Purkinje plate (PP) emerges in the normal (C) but not in reeler (D) cerebellum, and this initial difference became progressively enhanced until the perinatal period (E18.5), when the subpial layering versus deeply clustered patterns became complete. (E) Histogram showing the distance of green fluorescent protein (GFP) and Lhx1/5 double positive cells from the ventricular surface at the indicated stages (bottom) following Lyn-Venus adenovirus injections at E10.5, E11.5, and E12.5. Consistent with a general tendency that cells born earlier migrate further, the cells that contributed best to the emergence of the PP at E14.5 were born on E10.5 (presented separately at the top of the graph). Note, however, that some of the E10.5-born cells are seen deeply (within 150 μm from the ventricular surface) even at E15.5 and E16.5.