Figure 8
From: Nolz1 promotes striatal neurogenesis through the regulation of retinoic acid signaling
Striatal cultures are temporarily competent to regulate Nolz1 expression in response to RA. NPC and primary cultures were treated with RA and the expression of Nolz1 and RARβ were analyzed by Q-PCR. (A,B) RA increases the expression of Nolz1 and RARβ in a dose-dependent manner (0, 10-9, 10-8 and 10-6 M) in E12.5-derived neurospheres. (C,D) Within E14.5-derived neurospheres, RA increases the expression of RARβ in a dose-dependent manner (D) but it does not affect Nolz1 mRNA levels (C). (E) RA increases the levels of RARβ without affecting Nolz1 mRNA levels in LGE primary cultures treated with 10-8 M RA during 3 DIV. The results represent the mean ± standard error of the mean from at least three independent samples at each condition. Statistical analysis was performed with one-way ANOVA, followed by the Bonferroni post-hoc test (A-D) or with the Student's t-test (E). *P < 0.05, **P < 0.01, ***P < 0.001 relative to control.