Figure 5
From: Nolz1 promotes striatal neurogenesis through the regulation of retinoic acid signaling
Nolz decreases NPC proliferation in the SVZ of LGE organotypic cultures. (A) Organotypic cultures from E15.5 LGE were electroporated in the VZ/SVZ with hNolz (hNolz-DsRED) or the control vector (DsRED) and stained for Ki67 (white arrows indicate the fluorescent DsRED-expressing cells). Over-expression of hNolz produces a reduction in cell proliferation in the SVZ as shown by the reduction in Ki67-positive cells in the electroporated area. Scale bar: 600 μm. (B) High magnification of the electroporated area showing the decrease in Ki67-positive cells in the SVZ of the LGE. Scale bar: 300 μm. (C) Quantification of the number of Ki67-positive cells in the transfected area. The results represent the mean ± standard error of the mean from four independent experiments. Statistical analysis was performed with the Student's t-test; *P < 0.05.