Figure 3
From: Nolz1 promotes striatal neurogenesis through the regulation of retinoic acid signaling
Nolz1 is highly expressed in proliferating NPCs and down-regulated during differentiation. (A) Nolz1 mRNA expression is down-regulated during NPC differentiation. NPC samples from different stages of differentiation (0, 3 and 6 DIV) were analyzed by RT-PCR to study the expression pattern of Nolz1. The results are expressed as the percentage of the expression levels at 0 DIV, considered as 100%, and represent the mean from at least three independent samples at each condition. Error bars represent the standard error of the mean. Statistical analysis was performed with one-way ANOVA, followed by the Bonferroni post-hoc test. **P < 0.01, ***P < 0.001 relative to 0 DIV. (B) Nolz1 fluorescent immunocytochemistry was performed on NPC samples at different stages of differentiation (0, 3 and 6 DIV). Many Nolz1-positive cells were detected in proliferating neurospheres while the intensity and number of positive cells clearly decreased during differentiation. Scale bar: 50 μm.