Figure 16
From: Nolz1 promotes striatal neurogenesis through the regulation of retinoic acid signaling
Tle4 and Nolz1 expression show similar patterns in the developing LGE and in NPCs. (A) In situ hybridization study to analyze Tle1, Tle2, Tle3 and Tle4 mRNA levels in the LGE at E14.5. Dashed lines mark the VZ-SVZ boundary. (B) Simultaneous Tle4 immunohistochemistry and Nolz1 in situ hybridization show coincident expression patterns in the LGE at E14.5. (C) High levels of Tle4 mRNA are observed in proliferating NPCs (0 DIV), which decrease after differentiation (3 and 6 DIV). The results are expressed as the mean from at least three independent samples for each condition, and normalized to the proliferating NPCs (0 DIV), considered as 100%. Error bars represent the standard error of the mean. Statistical analysis was performed with one-way ANOVA, followed by the Bonferroni post-hoc test. **P < 0.01 relative to control. Scale bars: 600 μm (A); 100 μm (B).