Figure 14
From: Nolz1 promotes striatal neurogenesis through the regulation of retinoic acid signaling
RAR activity is essential for Nolz1 -mediated neurogenesis. (A,B) Nolz1 does not induce neuronal differentiation in the presence of a RAR inverse agonist. (A) Representative pictures of immunofluorescence for Tuj1 or GFAP after transfection of hNolz in the absence or presence of a RAR inverse agonist (BMS493). White arrows show double positive cells, open arrows show single EGFP stained cells. Scale bar: 30 μm. (B) The presence of the RAR inverse agonist (10-8 M BMS493) impairs the increase of Tuj1 and the reduction of GFAP observed after hNolz transfection in primary striatal cultures (Cont.). The results are expressed as the mean ± standard error of the mean from at least three independent samples for each condition, and normalized to the transfection in standard culture conditions (Cont.), considered as 100%. Statistical analysis was performed with the Student's t-test. **P < 0.01, ***P < 0.001 relative to control.