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Figure 1 | Neural Development

Figure 1

From: Nolz1 promotes striatal neurogenesis through the regulation of retinoic acid signaling

Figure 1

Characterization of the anti-Nolz1 polyclonal antibody. (A) Representation of Nolz1 showing the peptide against which the polyclonal antibody was raised. FKPY, Groucho consensus binding site; ZF, zinc-finger domain. (B) Western blots of NPC protein extracts, showing endogenous Nolz1 expression. Membranes were incubated with a rabbit serum obtained before (pre-serum; PS) or after (Nolz1) immunization. The specific band corresponding to Nolz1 (63 kDa; arrow) is visible only with the anti-Nolz1 antibody. (C) Dot-blot against Nolz1 pure peptide (upper drop; open arrowheads) or Keyhole limpet hemocyanin (KLH)-conjugated Nolz1 peptide (lower drop; closed arrowheads) incubated with rabbit serum before (pre-serum; PS) and after (Nolz1) immunization, showing Nolz1 antibody specificity. (D) Nolz1 pure peptide dots immuno-blotted with the anti-Nolz1 polyclonal antibody pre-incubated in the presence (Peptide) or in the absence (Control) of Nolz1 peptide.

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