Figure 3

At E15, axons expressing NRP-1 continue to distinctly compartmentalize in the olfactory nerve. Sagittal sections of CD1 embryos. (A-C') NRP-1 (green)/NCAM (red)/DRAQ5 (blue). (A) Lateral section. The open arrow indicates lack of segregation in fascicles targeting the caudal nerve. (B) Lateral-medial section. (C) Medial section. The open arrowhead shows the lack of segregation in rostral fascicles coming up to join the nerve. (C') High magnification of boxed region in (C). An orientation compass is shown in (A). Scale bars in (A-C) = 50 μm, C' = 10 μm.