Dose-dependent effect of T
on PC dendritic differentiation in organotypic cultures. (A-H). Organotypic cultures of P0 cerebella were kept 7 DIV in the absence of T3 (A,B), or in the presence of 3 nM T3 (C,D), 30 nM T3 (E,F) or 100 nM T3 (G,H). (A,C,E,G) PCs were revealed by CaBP immunolabeling. (B,D,F,H) Quantitative distribution of PCs between stages I to IV. Fusiform PCs with a bipolar shape are defined as stage I (arrow in (A,C)), PCs with regressive atrophic dendrites all around the soma are defined as stage II (white arrowhead in (A,C)), PCs with one or more perisomatic protrusions are defined as stage III (empty arrow in (G)) and PCs with an identified dendritic tree are classified as stage IV (empty arrowhead in (E)). Scale bar = 20 μm. Error bars indicate mean ± standard deviation.