Figure 7
Expression of dominant-negative RhoA, MRLC and constitutively active MRLC in chick hindbrains causes BM axon pathfinding errors. (A-N) Flat-mount chick hindbrains that have been electroporated with plasmids encoding myristylated GFP (myr-GFP), dominant-negative RhoA (DN-RhoA), dominant negative MRLC (DN-MRLC) and constitutively active MRLC (CA-MRLC) as labelled (note that (A-D) are myr-GFP controls). Immunostaining was with anti-GFP (green) and anti-Islet1/2 (blue) antibodies; (A) also immunostained using anti-SC1 antibodies (red). Rhombomeres are labelled. Yellow arrows show borders of the floor plate. White dotted lines show rhombomere boundaries. Yellow dotted lines show dorsal limit of exit point. White arrowheads indicate axon pathfinding errors. Scale bar: (A,B) 200 μm; (E,F) 100 μm; (G,H,K,L) 25 μm; (H,I,K) 15 μm; (C,D,J,M,N) 50 μm.