Figure 5

Characterization of fate-mapped Nkx2-1- and Shh -lineage and Shh -responder cells in the postnatal medial posteroventral nucleus. (A-L) P22 coronal sections of the MePV from Nkx2-1-Cre; Tau^mGFP(A,D,G,J), Shh^Cre; Tau^mGFP(B,E,H,K) and Gli1^CreER(T2); Tau^mGFP(TM E9.5: P22) (C,F,I,L) brains showing dual immunofluorescence for β-galactosidase (β-gal⁺) recombined cells (green) and the expression of the inhibitory neuronal markers Calbindin (top row), neuronal nitric oxide synthase (nNOS; second row), FoxP2 (third row) and Somatostatin (last row). (A'-H') High-power images from corresponding boxed regions in (A-L) also show DAPI nuclear counterstaining (blue) in single optical sections from confocal images. Co-expressing cells (arrows), β-gal⁺/marker^- (closed arrowheads) and β-gal^-/marker⁺ (open arrowheads) cells are indicated. (M-O) Pie charts depicting the percentage value of β-gal⁺ cells in each Cre line that co-expressed the corresponding neuronal marker. The images were taken from either the rostral or caudal portions of the posterior MeA in which the analysis was performed. Abbreviations: opt, optic tract. Scale bar: 200 μm (A-L); 100 μm (A'-L').