Figure 1

Short-term recombination in Shh^Creand inducible Gli1^CreER(T2)lines. (A-D) Rostro-caudal sequence from coronal sections of E12.5 Shh^Cre; Tau^mGFPbrains (n = 2), where LacZ staining reveals recombined cells in the region of the MGE, POA and ventral telencephalon (vTEL) (arrows) and, to a lesser extent, in the thalamus (TH), cortex (CTX) and hippocampus (HP). (E-H) A single dose of tamoxifen (TM) administered at E9.5 labeled cells at approximately E10.5 in Gli1^CreER(T2); Tau^mGFPbrains (n = 2). The inducible Gli1^CreER(T2)line was used to identify Shh-responding cells during a temporally restricted time window. A rostro-caudal sequence shows LacZ⁺ cells in the POA and vTEL (arrows). Adjacent sections, processed for Foxg1 mRNA expression (insets), show that the recombined cells are in telencephalic domains of the forebrain. Abbreviation: LGE, lateral ganglionic eminence. Scale bar: 150 μm.