Figure 7

Zfhx1b over-expression is not sufficient to alter proliferation of definitive NSCs derived from the 14.0-dpc striatal germinal zone. (A) Images of control (EGFP) transfected or Zfhx1b + EGFP transfected 14.5-dpc striatal germinal zone cells incorporating BrdU (red) with DAPI (blue) nuclear labeling (scale bar: 50 μm). (B) The percentage of BrdU+ cells in the control or EGFP + Zfhx1b transfected cultures was not significantly different (P = 0.35); cultures plated in duplicate from n = 4 separate tissue samples in each group. (C) DIC and EGFP images of NSC colonies derived from 14.5-dpc striatal germinal zone cultures transfected with either control (EGFP) or Zfhx1b + EGFP constructs (scale bar: 50 μm). DIC, differential interference contrast. (D) The number of FGF + H derived colonies from either the control or Zfhx1b transfected cultures was not significantly different (P = 0.51). The results represent the average from two separate experiments; n = 3 embryos per treatment group and four replicates per culture condition in each experiment. S.d., standard deviation.