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Figure 5 | Neural Development

Figure 5

From: FGF dependent regulation of Zfhx1b gene expression promotes the formation of definitive neural stem cells in the mouse anterior neurectoderm

Figure 5

The Zfhx1b gene is modulated by FGF and Wnt signaling and is sufficient to rescue FGF-responsive neural stem cell colonies in the presence of SU5402. (A) Expression profile of Zfhx1b by whole mount in situ hybridization. Arrow, anterior ectoderm; EE, extraembryonic ectoderm. (B) Modulation of FGF and Wnt signaling alters the expression of Zfhx1b as shown by quantitative RT-PCR. Tissue samples prepared as in Figure 1. (C) Pre-treated 8.5-dpc anterior neural plate cells were transfected with an empty vector (EGFP) or the Zfhx1b over-expression construct and cultured in serum-free media for 14 days (three separate experiments; n = 2 to 3 embryos for each treatment group; two replicates per culture condition per treatment). *Statistically significant (P < 0.05) compared to the transfection control EGFP transfected in either culture conditions; ** statistical significance (P < 0.05) of the respective transfection in the two culture conditions, FGF2 + heparin (H) or LIF. S.d., standard deviation.

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Neural Development

ISSN: 1749-8104