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Figure 3 | Neural Development

Figure 3

From: FGF dependent regulation of Zfhx1b gene expression promotes the formation of definitive neural stem cells in the mouse anterior neurectoderm

Figure 3

FGF receptor and MAPK signaling inhibition maintains pNSC identity and prevents the transition to a NSC identity in the anterior ectoderm. (A) Inhibition of FGF receptor using SU5402 (five separate experiments; n = 2 embryos for each treatment group; four replicates per culture condition in each experiment). (B) Analysis of phospho-Erk1/2 levels in the presence of FGF8b or SU5402, compared to total Erk1/2 protein levels. (C) Similar experiment using the MEK inhibitor, U0126 (three separate experiments; n = 2 to 3 embryos for each treatment group; four replicates per culture condition per treatment in each experiment). *Statistical significance compared to the positive control dimethyl sulfoxide (DMSO) injected, FGF2 + heparin (H) cultured, P < 0.05; **statistical significance compared to DMSO injected, LIF cultured, P < 0.05. (D) Phospho-Erk1/2 levels in the presence of U0126 compared to total Erk1/2 protein levels. S.d., standard deviation.

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Neural Development

ISSN: 1749-8104