Figure 3

Rapid disappearance of Glu immunoreactivity from the mushroom body α/β cores following adult eclosion. (A-C) Transverse sections of the MB pedunculus from 17d-GAL4 (A), c739-GAL4 (B) and 201Y-GAL4 (C) adult flies expressing mCD8::GFP and labeled for Glu at different ages. Similar results were obtained with the three driver lines. (A-C, a1-a3) Within 1 hour after eclosion, the core neurons express Glu but not GFP. Only thin processes in the outer layers of the pedunculus are positive for both. (A-C, b1-b3) Twenty-four hours after eclosion, the intensity of Glu labeling already dramatically decreased while the expression of GFP started to appear in the core neurons. (A-C, c1-c3) Ten days after eclosion, there is no detectable Glu in the core neurons and GFP is strongly expressed in these cells. Scale bars: 10 μm.