Figure 2

Commonly used mushroom body drivers do not target the last born Kenyon cells just after adult eclosion. (A) In 17d-GAL4; UAS-mCD8::GFP adults just after eclosion, GFP is not present in the inner axons of the α/βc neurons as seen in the lobe (arrow) or in the pedunculus (arrowheads in the insert). (B) Ten days later, all the α/βc KCs express GFP as shown in the lobes and pedunculus (insert). (C, D) Similar observations with the c739-GAL4 driver line that does not express GFP in the α/βc neurons right after eclosion (C), but target all α/β KCs 10 days later (D). sp, spur. (E, F) Similar observations with the 201Y-GAL4 driver line that targets both the α/β and γ neurons. This line does not target the last born α/βc KCs just after eclosion as well. Scale bars: 20 μm.