Figure 3

MARCM clones cluster ventrolateral to the antennal lobes. (A) Two-cell clones (yellow) and three- to five-cell clones (red) from single heat-shock animals (n = 12 brains) are found ventrolateral to the antennal lobes. The black arrow indicates a four-cell clone that is shown in (B). (B) A z-series of six consecutive, one micron optical sections, running from left to right. The four cells of the clone are marked by H2B-monomeric red fluorescent protein (mRFP) and are outlined with dashed white lines. The white arrows indicate three other single cell clones. Discs large (DLG; blue) outlines cell cortices and the neuropile. AL, antennal lobe; AN, antennal nerve; VLPR, ventrolateral protocerebrum. The scale bar is 20 μm.