Hierarchal clustering of the dataset reveals the level of genetic relatedness between rhombomeres. Data sourced from the validated dataset shows that rhombomere 2 (r2; Hoxa2) and r4 (Hoxa2, Hoxb2, Hoxb1) are more related to each other than the r3 (Hoxa2, Egr2) and r5 (Hoxa2, Egr2, MafB) territories. These findings are consistent with previously described expression patterns and other data examining the cellular behaviour of these territories. Each gene in the cluster is represented by a single box at the same level and relative expression values in each rhombomere are depicted by the actual colour. Blue = underrepresented; yellow = equivalent to; and red = enriched with respect to the median expression value of that gene in all samples. The confidence scores are reported adjacent to each branch.