Figure 6
From: Ascl1 is a required downstream effector of Gsx gene function in the embryonic mouse telencephalon
Gsx2;Ascl1 double mutants exhibit a more severe phenotype in the formation of the dorsal lateral ganglionic eminence (dLGE) and the generation of olfactory bulb interneurons. (A, B) The Sp8 expression domain in the dLGE is reduced in Gsx2 mutants (B) compared to controls (A). (D) Gsx2;Ascl1 double mutants exhibit a more severe reduction in the expression of Sp8 in the dLGE (D) compared to Gsx2 mutants (B). (C) Ascl1 mutants maintain Sp8 expression in the dLGE and may have a slightly expanded expression domain. (E, F) Sp8 expressing interneurons are reduced in Gsx2 mutant olfactory bulb (F) compared to controls (E). (H) Gsx2;Ascl1 mutant displays nearly a complete loss of Sp8 expression in the olfactory bulb. (G) Sp8 expression is observed in Ascl1 mutant olfactory bulbs, but in a slightly disorganized pattern (compare (G) to (E)). gl, glomerular layer.