Figure 5
From: Ascl1 is a required downstream effector of Gsx gene function in the embryonic mouse telencephalon
Gsx1 expands throughout the Gsx2;Ascl1 mutant lateral ganglionic eminence (LGE). (A-C) Gsx1 expression is barely present in the E16.5 wild type LGE (A) but appears to be expressed similarly in Gsx2 (B) and Gsx2;Ascl1 mutant (C) LGEs. (D-F) The Gsx1/2 antibody can be used in Gsx2 mutants to visualize Gsx1 protein expression and not only is gene expression expanded in the mutants compared to wild type (D) but Gsx1 protein is found in a similar pattern in the Gsx2 mutant (E) and Gsx2;Ascl1 mutant (F) LGEs. The Gsx1/2 staining in the wild type mostly reflects Gsx2 expression since very little Gsx1 expression in seen in the wild type LGE (A).