Figure 3
From: Ascl1 is a required downstream effector of Gsx gene function in the embryonic mouse telencephalon
Increase in progenitor cell markers in the lateral ganglionic eminence (LGE) of Ascl1 mutants. (A, D) At E12.5, Ascl1 mutants express Gsx2 in a relatively normal pattern in the LGE compared to wild type. Note the odd morphology in the developing medial ganglionic eminence (MGE) of Ascl1 mutants (asterisk in (D)). (B, E) By E18.5, Gsx2 protein expression is increased in the entire LGE region of Ascl1 mutants (E) compared to controls (B). These ectopic Gsx2 positive cells are observed in the SVZ and striatum, many appearing as clumps of cells stuck in the parenchyma (arrow in (E)). (C, F) Dividing cells labeled by Ki67 expression are also increased in the Ascl1 mutant LGE area (F) compared to control (C). Similar to Gsx2, many ectopic Ki67 positive cells appear as clumps (arrow in (F)).