Figure 1
From: Ascl1 is a required downstream effector of Gsx gene function in the embryonic mouse telencephalon
Knock-in of enhanced green fluorescent protein (EGFP) into the Gsx2 locus. (A, B) EGFP expression in Gsx2EGFP/+embryos (A) recapitulates endogenous Gsx2 expression at E12.5 (B). (C) Note that EGFP expression persists longer than Gsx2 protein expression in the lateral ganglionic eminence (LGE; merged image). – (D) The Gsx1/2 antibody detects expression of Gsx1 and Gsx2 in the ventral telencephalon. (E) Note that the EGFP expression is absent in the septal expression domain of the Gsx1/2 antibody (asterisks in D, E, I, J) indicating Gsx1-specific expression. (F-H) Homozygous knock-in of EGFP into the Gsx2 locus (Gsx2EGFP/EGFP) (F) results in a loss of function of Gsx2 with no detectable protein expression (G). Note that the EGFP expression is more intense in the Gsx2EGFP/EGFPembryos (H) compared to the Gsx2EGFP/+embryos (A). (I) The initial expansion of Gsx1 into the LGE of Gsx2EGFP/EGFPembryos is detectable at E12.5 with an anti-Gsx1/2 antibody. (J) Gsx1 expression is not observed in all Gsx2 mutant cells of the LGE (merged image) but found largely at the VZ/SVZ boundary (arrows in I, J).