Projection pattern of the larval DM4 lineage. (A-C) Consecutive confocal images (from dorsal to ventral) of DM4 MARCM-labeled clone (GFP, green). Secondary neurons and their projections are stained with anti-Neurotactin (Nrt, magenta). Note commissural (arrows) and longitudinal (arrowheads) projections. (D) Z-projection of the entire DM4 lineage. Brain and oesophagus outline shown as a magenta dashed line; midline is a white dashed line. (E, F) A close-up view of the commissural projections. Note the site where DM4 axons enter the commissure (asterisk). Although not visible in this figure, a subset of the commissural axons enters the contralateral brain hemisphere.