Projection pattern of the larval DM1 lineage. (A-C) Consecutive confocal images (from dorsal to ventral) of DM1 MARCM-labeled clone (GFP, green). Secondary neurons and their projections are stained with anti-Neurotactin (Nrt, magenta). Note commissural (arrows) and longitudinal (arrowheads) projections. (D) Z-projection of the entire DM1 lineage. Brain and oesophagus outline shown as a magenta dashed line; midline is a white dashed line. (E, F) A close-up view of the commissural projections. Note how the axonal bundle defasciculates (arrows) upon entering the commissure and that a subset of these axons enters the contralateral brain hemisphere. Also note that DM1 axons enter the commissure at a most medial site (asterisk).