Example of a second DM lineage projection pattern in the pupal brain 24 hours after puparium formation. (A-C) Consecutive Z-projections prepared from transverse confocal sections of pupal brain neuropile. Panels show both brain hemispheres; (A) is the most dorsal, and (C) the most ventral section, with the midline in the center and anterior part pointing up (neuraxis). DM MARCM clone is labeled with anti-GFP (green); brain neuropile is labeled with anti-nc82 (magenta). Note the contralateral (arrows) and ipsilateral (arrowheads) projections. (D) A close-up view of the major ipsilateral arborization. (E, F) A close-up view of the projections into the developing fan-shaped body and contralateral nodulus. Note two fascicles (arrowheads in (F)) entering the fan-shaped body and forming columnar arborizations. Also note the arborization formed in the contralateral nodulus (arrow in (F)). Dashed lines indicate the outlines of the fan-shaped body and noduli.