Figure 5
From: Multiple non-cell-autonomous defects underlie neocortical callosal dysgenesis in Nfib-deficient mice
Diminished expression of GLAST at the cortical midline of Nfib -deficient mice. (A-F) Expression of GLAST at E14 (A, B), E16 (C, D) and E18 (E, F) in coronal sections of wildtype (A, C, E) and Nfib knockout (B, D, F) brains. At E14 in the wildtype (A), GLAST was expressed in the glial wedge (arrow), and this expression intensified as development proceeded (arrows in (C, E)). Furthermore, GLAST expression was observed in the indusium griseum glia (arrowhead) and midline zipper glia (double arrowhead) at E18 in the wildtype (E). In the mutant, however, GLAST expression in the glial wedge was reduced in comparison to controls (open arrowheads; compare (B) to (A), and (D) to (C)). Moreover, the indusium griseum glia and midline zipper glia in the Nfib null mutant were not apparent in the mutant via GLAST immunohistochemistry at E18 (F). Scale bars: 300 μm (A, B); 250 μm (C, D); 200 μm (E, F).