Figure 4
From: Multiple non-cell-autonomous defects underlie neocortical callosal dysgenesis in Nfib-deficient mice
Radial progenitor cells express nestin at higher levels at E18 in Nfib mutant mice. (A-F) Coronal sections of wildtype (A, C, E) and Nfib-deficient brains (B, D, F) demonstrating expression of nestin. At E14 (A, B), E16 (C, D) and E18 (E, F), expression of nestin in the mutant was comparable to that in the control. (G) At E18, levels of nestin mRNA were significantly higher in Nfib mutants than in littermate controls (*P < 0.05; t-test). RNA from three independent replicates for both wildtype (WT) and Nfib mutants (Nfib knockout (KO)) was analysed. Error bars indicate standard error of the mean. Scale bar: 300 μm (A, B); 250 μm (C, D); 200 μm (E, F).