Figure 2
From: Multiple non-cell-autonomous defects underlie neocortical callosal dysgenesis in Nfib-deficient mice
Reduced expression of GFAP in Nfib knockout mice. (A-H) Expression of GFAP at the cortical midline of wildtype (A, C, E, G) and Nfib-deficient (B, D, F, H) mice. In the wildtype, expression of GFAP in the glial wedge was initiated at E15, and became progressively stronger as development proceeded (arrows in (A, C, E, G)). GFAP expression in the indusium griseum glia (arrowhead) and midline zipper glia (double arrowhead) of the wildtype was also evident from E17 onwards (E, G). However, in the mutant, low levels of GFAP expression in the glial wedge were only observed from E17 onwards (open arrowheads in (F, H)), whereas expression in the indusium griseum glia and midline zipper glia was absent. Neurons in the indusium griseum of the wildtype expressed Tbr1 (arrows in (I)). Neurons expressing Tbr1 in the indusium griseum were also observed in the mutant (arrows in (J)). Scale bars: 280 μm (A, B); 250 μm (C, D); 225 μm (E, F); 200 μm (G-J).