Skip to main content
Figure 1 | Neural Development

Figure 1

From: Role of the Wnt receptor Frizzled-1 in presynaptic differentiation and function

Figure 1

Fz1 is located at presynaptic sites in hippocampal neurons. (A) Immunodetection of Fz1 in hippocampal neurons maintained for 14 days in culture shows a clustered distribution of the receptor (scale bar = 10 μm). (B) Representative merged images with the postsynaptic marker PSD-95 and the presynaptic protein synapsin I (Syn) indicate that Fz1 clusters are located presynaptically in close apposition to PSD-95 puncta. (C) Overlap coefficient according to Manders et al. [17] indicates an overlap of Fz1 and Syn signals. (D) Synaptic vesicles were labeled by FM1-43 uptake in hippocampal neurons transfected with GFP. After fixation and permeabilization, cells were inmunostained with anti-VAMP and anti-Fz1 and the same GFP-positive neuron was analyzed by confocal microscopy. As observed, Fz1 is present in most of the presynaptic terminals stained with FM1-43 and VAMP (arrows; scale bar = 10 μm). Error bars indicate standard error of the mean. **P < 0.01.

Back to article page