Figure 5

Immunoprofiling of cortical periventricular layers after in utero electroporation of plasmids pPri-miR-124(2) and pPri-miR-155/neg_control; part II. (A, B, D, E) Distribution of β-tubulin, Tbr1 and pCMV-driven EmGFP on E14.5 mid-frontal sections from brains electroporated in utero at E12.5 with plasmids pPri-miR-124(2) and pPri-miR-155/neg_control. In (A, D), arrowheads point to periventricular cells expressing β-tubulin and Tbr1, respectively. (C) Comparison of pCMV-driven EmGFP and neuron-specific β-tubulin with miR-124 expression in the electroporated area shown in (A, B, D, E). Magnifications of boxed insets to the right show an EmGFP⁺ electroporated cell co-expressing huge amounts of miR-124 and β-tubulin (arrow), as well as another EmGFP⁺/miR-124⁺ cell negative for β-tubulin (asterisk). Electroporated zones shown throughout Figure 5 correspond to the boxed areas in the schematics of (A, B). Scale bars = 100 μm.